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His friends, who are also his neighbors, include down- and- out divorced Army barber Bill Dauterive, cuckolded Conspiracy Theorist and pest exterminator Dale Gribble, and Boomhauer, a womanizing motormouth whose job was unknown until a last- second reveal in the series finale. His family includes his wife, Peggy (a Small Name, Big Ego if there ever was one, at least in the later episodes; the earlier ones had her being a little more sane), his son, Bobby (who, according to Hank, is . Hank also has plenty of conflicts with his father, Cotton, a misogynistic World War II veteran (who had both of his shins blown off by a Japanese soldier during an ambush). One thing that stands out the most about the series is that unlike most other animated sitcoms that feature wacky or outlandish situations (i. 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Link: https://www.facebook.com/arthurcristian/posts/10209990133778004. FOX episodes). Netflix streaming in America used to have every episode of the show (including the four unaired ones) until they were pulled in 2. The show has a character sheet. It's an important plot line that runs throughout Season 3 and is mentioned in the first episode in Season 4. Then, it's dropped completely, though it should be noted, that this plot line ended with Peggy being paralyzed in a skydiving accident and requiring months of painful rehabilitation. A CONNECTICUT YANKEE IN KING ARTHUR'S COURT A WORD OF EXPLANATION It was in Warwick Castle that I came across the curious stranger whom I am going to. Luanne going to college after meeting Buckley's angel in Season 3. In Season 8, she drops out of college and goes back to hairstyling, despite Buckley's angel telling her she was meant for something better. Technically, this is a Retcon but it also aborts the ongoing arc of Luanne getting away from her trailer trash origins. This is especially the case once she hooks up with Lucky, who Peggy points out represents the life from which Luanne was originally trying to escape (though, technically, Luanne isn't trailer trash. She doesn't live in a trailer.. The only reason people think she's become trailer trash is because Lucky is a redneck). Above the Influence: Boomhauer, when Luanne gets dumped and stays at his house. She thinks he's hitting on her, only for him to put a pillow and blanket on the couch and walk away. Accidental Misnaming: In . Seems to me that boy does have a hero, and his name is Hank Hall. Accidental Pervert: The main plots of . What if I have nothing in common with my kid? What if my kid gets bullied for being different? What if my spouse cheats on me? What if my spouse leaves me? All of the main characters face very real problems. Advice Backfire: . Late in the game, the Nebraska coach calls the box to ask for advice and Hank, pretending to be the player, gives him a terrible suggestion, only for it to improbably work perfectly, costing Texas the game. Aesop Amnesia: Hank has learned to forgive Bobby's lack of athleticism and appreciate his other skills about 3. Maybe it's genetic, as Hank has earned the grudging respect of his father Cotton on several occasions, and that never stuck, either. No matter how many times Hank learns to loosen up, this still happens a lot: Bobby: Hey Dad, guess what? I joined the (insert incredibly effeminate and/or non- traditional activity here)! Hank constantly forgets that Bobby is good at some sports, like shooting, football, and wrestling. Good examples of this are when he learned that she repeatedly took odd jobs to get out of the house and that her miniature collection was literally her Sanity Ball. Kahn and Minh quite frequently learn to respect their redneck neighbors only to forget it. Quintessential to this is Buck Strickland, who fails to learn that his illegal schemes will put his business at risk, and Hank, who worships Strickland, never gets an Aesop that his boss is an immoral jackass and that he'll always get in trouble for trying to clean up after his boss's schemes. When Kahn becomes a hillbilly, he shows himself to be quite proficient at a distinctly non- hillbilly fighting style, besting a significantly larger man. This results in him being invited to . Careful observers will note that Kahn is using Pencak Silat. All Germans Are Nazis: Mentioned by Bobby after Cotton tells Bobby's class how he garroted a German soldier with dental floss: Connie: So did you kill the German corporal? Bobby: *snort* This was World War II, Connie. He was a Nazi! All Girls Want Bad Boys: In a hilariously lame version of this, Peggy seems to get turned on when Hank starts driving a secret lunch truck after Arlen bans transfat in ? In actuality, this was disturbingly real punishment for years. An Aesop: Lampshaded in ? Because they're both equally valid. The moral, incidentally, is . Most episodes, invariably, have this in some form or another, usually delivered by Hank. Ambiguously Gay: When Peggy joins the PTA, one of the board members acts like a flaming gay stereotype.. This becomes a running gag through all of his appearances - notably in Strangeness on a Train, where he seems to be . Also his memorable line: ? Also, the costumes for the working wenches exposed too much skin, and King Philip's outfit looks more like a medieval outfit. Not to mention there was never a King Philip of England. Peggy attempts to correct some of the inaccuracies, and that is the start of Philip disliking her. Annoying Laugh: Kahn has an annoying, condescending one. He can't sway the oversight board into believing him. He then bring the yoga instructor who helped him as a witness. When the instructor acts obnoxious, Hank points out that he would never spend time with a guy like this unless he absolutely had to. This is enough to convince the board that Hank is telling the truth. Aren't You Going to Ravish Me?: When Luanne stays over at Boomhauer's for a few nights, she assumes it is a pretext for sex and tries (unconvincingly) to let Boomhauer down gently, and is then bewildered when he puts a blanket and pillow on the couch and goes to his bedroom. Arson, Murder, and Jaywalking: Dale: I have dedicated my professional career to the study and control of arthropods. I have personally taste- tested each and every household insecticide. I have read a book. Art Evolution: During Season 1, there's a small but noticeable improvement after the pilot episode. Hank got a few less facial wrinkles starting in Season 2, and his glasses shrunk down. Also, Peggy's tank top switched from gray to green starting in Season 2, along with no longer wearing tall socks. And Bobby's face was altered to be more cheerful and not look like he's sullen and/or mentally retarded. The colors also became brighter and less washed out. Later, when Joseph's voice actor was recast, he was made to go through a growth spurt, complete with facial hair, a deeper voice, and new clothes. Later seasons switched from ink animation to digital animation and some occasional CGI mixed with 2. D (as seen on Futurama, Family Guy, and The Simpsons). The typeface of signs suffered from this switch, as they no longer looked blended in with the animation. Artifact Title: . Only the first act ties into the episode title (as it centers on Hank getting in trouble for mooning while in a glass elevator); the rest is about Bill's relationship with the former governor of Texas, Ann Richards. Artistic License . In reality, Ontario lacks mountains. On a related note, the idea that Boomhauer would hook up with a French- speaker in Guelph, Ontario, while obviously not a logical impossibility any more than meeting one in Arlen, is extremely improbable given the demographics, and seems more the product of the writers thinking that all of Canada is bilingual when really, it's just New Brunswick and a select number of Quebec cities (Montr. The safety instructor for Bobby's class explains that he lost an eye and a thumb by excitedly running downrange to show off his target (presumably he got the job for being a walking example of how not to act on a range). He later repeats this when he sees Bobby's target, while Bobby is more concerned with PROVEing his rifle safe. Artistic License . Most were hanged, and one was crushed under stones. Could be a deliberate error to highlight the character's ignorance, as most people in real life do believe that women accused of witchcraft were burned during the Salem Witch Trials. Artistic License . First Arlen Methodist displayed in one episode the trademarked logo of the United Methodist Church. Its disappearance in other episodes afterwards leads one to believe that the show mistakenly believed it was a generic Methodist logo. Cloning - Wikipedia. Many organisms, including aspen trees, reproduce by cloning. In biology, cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. The term also refers to the production of multiple copies of a product such as digital media or software. The term clone, invented by J. Haldane, is derived from the Ancient Greek word . In horticulture, the spelling clon was used until the twentieth century; the final e came into use to indicate the vowel is a . It is the reproduction method used by plants, fungi, and bacteria, and is also the way that clonal colonies reproduce themselves. Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non- coding sequences and randomly fragmented DNA. It is used in a wide array of biological experiments and practical applications ranging from genetic fingerprinting to large scale protein production. Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning. In practice, localization of the gene to a chromosome or genomic region does not necessarily enable one to isolate or amplify the relevant genomic sequence. To amplify any DNA sequence in a living organism, that sequence must be linked to an origin of replication, which is a sequence of DNA capable of directing the propagation of itself and any linked sequence. However, a number of other features are needed, and a variety of specialised cloning vectors (small piece of DNA into which a foreign DNA fragment can be inserted) exist that allow protein production, affinity tagging, single stranded RNA or DNA production and a host of other molecular biology tools. Cloning of any DNA fragment essentially involves four steps. Subsequently, a ligation procedure is used where the amplified fragment is inserted into a vector (piece of DNA). The vector (which is frequently circular) is linearised using restriction enzymes, and incubated with the fragment of interest under appropriate conditions with an enzyme called DNA ligase. Following ligation the vector with the insert of interest is transfected into cells. A number of alternative techniques are available, such as chemical sensitivation of cells, electroporation, optical injection and biolistics. Finally, the transfected cells are cultured. As the aforementioned procedures are of particularly low efficiency, there is a need to identify the cells that have been successfully transfected with the vector construct containing the desired insertion sequence in the required orientation. Modern cloning vectors include selectable antibiotic resistance markers, which allow only cells in which the vector has been transfected, to grow. Additionally, the cloning vectors may contain colour selection markers, which provide blue/white screening (alpha- factor complementation) on X- gal medium. Nevertheless, these selection steps do not absolutely guarantee that the DNA insert is present in the cells obtained. Further investigation of the resulting colonies must be required to confirm that cloning was successful. This may be accomplished by means of PCR, restriction fragment analysis and/or DNA sequencing. Cell cloning. Cloning unicellular organisms. Cloning cell- line colonies using cloning rings. Cloning a cell means to derive a population of cells from a single cell. In the case of unicellular organisms such as bacteria and yeast, this process is remarkably simple and essentially only requires the inoculation of the appropriate medium. However, in the case of cell cultures from multi- cellular organisms, cell cloning is an arduous task as these cells will not readily grow in standard media. A useful tissue culture technique used to clone distinct lineages of cell lines involves the use of cloning rings (cylinders). At an early growth stage when colonies consist of only a few cells, sterile polystyrene rings (cloning rings), which have been dipped in grease, are placed over an individual colony and a small amount of trypsin is added. Cloned cells are collected from inside the ring and transferred to a new vessel for further growth. Cloning stem cells. Somatic- cell nuclear transfer, known as SCNT, can also be used to create embryos for research or therapeutic purposes. The most likely purpose for this is to produce embryos for use in stem cell research. This process is also called . While a clonal human blastocyst has been created, stem cell lines are yet to be isolated from a clonal source. The process begins by removing the nucleus (containing the DNA) from an egg cell and inserting a nucleus from the adult cell to be cloned. The reprogrammed cell begins to develop into an embryo because the egg reacts with the transferred nucleus. The embryo will become genetically identical to the patient. This process can either add or delete specific genomes of farm animals. A key point to remember is that cloning is achieved when the oocyte maintains its normal functions and instead of using sperm and egg genomes to replicate, the oocyte is inserted into the donor. The first step is to collect the somatic cells from the animal that will be cloned. The somatic cells could be used immediately or stored in the laboratory for later use. Once this has been done, the somatic nucleus can be inserted into an egg cytoplasm. The grouped somatic cell and egg cytoplasm are then introduced to an electrical current. The successfully developed embryos are then placed in surrogate recipients, such as a cow or sheep in the case of farm animals. It successfully cloned sheep, cattle, goats, and pigs. Another benefit is SCNT is seen as a solution to clone endangered species that are on the verge of going extinct. For example, the cloned sheep Dolly was born after 2. SCNT, which created 2. Only three of these embryos survived until birth, and only one survived to adulthood. The biochemistry involved in reprogramming the differentiated somatic cell nucleus and activating the recipient egg was also far from being well- understood. However, by 2. 01. The resulting hybrid cells retain those mitochondrial structures which originally belonged to the egg. As a consequence, clones such as Dolly that are born from SCNT are not perfect copies of the donor of the nucleus. Organism cloning. Organism cloning (also called reproductive cloning) refers to the procedure of creating a new multicellular organism, genetically identical to another. In essence this form of cloning is an asexual method of reproduction, where fertilization or inter- gamete contact does not take place. Asexual reproduction is a naturally occurring phenomenon in many species, including most plants (see vegetative reproduction) and some insects. Scientists have made some major achievements with cloning, including the asexual reproduction of sheep and cows. There is a lot of ethical debate over whether or not cloning should be used. However, cloning, or asexual propagation. Many horticultural plant cultivars are clones, having been derived from a single individual, multiplied by some process other than sexual reproduction. Other examples are potato and banana. Parts of an individual plant may become detached by fragmentation and grow on to become separate clonal individuals. A common example is in the vegetative reproduction of moss and liverwort gametophyte clones by means of gemmae. Some vascular plants e. This is an asexual form of reproduction that is only found in females of some insects, crustaceans, nematodes. The growth and development occurs without fertilization by a male. In plants, parthenogenesis means the development of an embryo from an unfertilized egg cell, and is a component process of apomixis. In species that use the XY sex- determination system, the offspring will always be female. An example is the little fire ant (Wasmannia auropunctata), which is native to Central and South America but has spread throughout many tropical environments. Artificial cloning of organisms. Artificial cloning of organisms may also be called reproductive cloning. First moves. Hans Spemann, a Germanembryologist was awarded a Nobel Prize in Physiology or Medicine in 1. In 1. 92. 8 he and his student, Hilde Mangold, were the first to perform somatic- cell nuclear transfer using amphibian embryos . This process entails the transfer of a nucleus from a donor adult cell (somatic cell) to an egg from which the nucleus has been removed, or to a cell from a blastocyst from which the nucleus has been removed. Such clones are not strictly identical since the somatic cells may contain mutations in their nuclear DNA. Additionally, the mitochondria in the cytoplasm also contains DNA and during SCNT this mitochondrial DNA is wholly from the cytoplasmic donor's egg, thus the mitochondrial genome is not the same as that of the nucleus donor cell from which it was produced. This may have important implications for cross- species nuclear transfer in which nuclear- mitochondrial incompatibilities may lead to death. Artificial embryo splitting or embryo twinning, a technique that creates monozygotic twins from a single embryo, is not considered in the same fashion as other methods of cloning. During that procedure, an donor embryo is split in two distinct embryos, that can then be transferred via embryo transfer. It is optimally performed at the 6- to 8- cell stage, where it can be used as an expansion of IVF to increase the number of available embryos. Dolly was formed by taking a cell from the udder of her 6- year old biological mother. It took 4. 34 attempts before an embryo was successful. 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